In this lecture, students will know the role of plant cell culture and how to development a process for bioactive compound (secondary metabolites and exogenious proteins) production. And students will learn the research methods concerned from bioactive compound screening to thus isolation & identification. Some important cases of plant cell culture in bioindustry and trend-development in plant biotechnology will also be included in this lecture.

Week Content
1 Introduction to plant biotechnology
2 Plant nutrition & phytohormones
3 Technique & instruments in plant cell culture
4 Screening methods for detection and evaluation of
biological activities of plant preparation
5 Extraction﹜isolation and identification of bioactive
compounds from medicinal plant
6 Plant secondary metabolism---flavonoids
7 Plant secondary metabolism---Taxus alkaloids and taxol
8 Invitation speech
9 Principle & application for plant bioreactor---(1)
10 Principle & application for plant bioreactor---(2)
11 Suspension cell culture
12 Hairy root culture
13 Plant organ culture
14 Plant cell as a factory for recombinant protein production
15 Antibody expressed in transgenic plants
16 Trends in plant biotechnology
17 Final report

Organic chemistry & Biochemistry are the basic programs for this lecture

1.Wink, M. (1999) Functions of plant secondary metabolites
and their exploitation in biotechnology. CRC Press, New York.
2.Seigler, D.S. (1998) Plant secondary metabolism. Kluwer Academic
Publisher, London.
3.DiCosmo, F. & Misawa, M. (1996) Plant cell culture secondary
metabolism. CRC Press, New York.
4.Verrall, M. (1996) Downstream Processing of Natural Products.
John Wiley & Sons Ltd, New York.
5.Bohlin, L. and Bruhn J.G. (1999) Bioassays methods in natural
product research and drug development. Kluwer Academic Publisher,
London.
6.Harper, K. and Ziegler, A. (1999) Recombinant antibodies. Taylor
& Francis, London.